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Image Search Results
Journal: Advanced Healthcare Materials
Article Title: 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and Functionalization
doi: 10.1002/adhm.202402606
Figure Lengend Snippet: Immunostaining for SSC markers following the replacement of the neonatal mouse SSC growth factor EGF with Akt‐inhibitor MK2206 in hiPSC‐SSC cultures.
Article Snippet: SSCs were expanded on a sulfated dextran‐4‐armed polyethylene glycol (starPEG) hydrogel matrix functionalized with vitronectin and fibronectin type II domain (FN2) peptide motifs (denovoMATRIX, GmbH) in Human Plasma‐Like Medium (HPLM), 15% Cell Therapy Systems (CTS) KnockOut Serum Replacement (SR) XenoFree supplement (Gibco, 12 618 012), 1 μM vitamin C (Millipore Sigma, A4403), 1 μM vitamin E (Millipore Sigma, T1157), 10 μg mL −1 biotin (Millipore Sigma, B4639), 60 ng mL −1 progesterone (Millipore Sigma, P8783), 60 μM putrescine (Millipore Sigma, P5780), 30 μg mL −1 pyruvate (Millipore Sigma, S8636), 30 ng mL −1 β‐estradiol (Sigma, E2758), 1X Minimum Essential Medium (MEM) Vitamin Solution (Gibco, 11 120 052), 1X GlutaMAX Supplement (Gibco, 35 050 061), 1 μL mL −1 DL‐lactate (Millipore Sigma, L4263), 50 μM β‐mercaptoethanol (Gibco, 31350‐010), 15 ng mL −1 Glial Cell‐Derived Neurotrophic Factor (GDNF, Peprotech, AF‐450‐010), 10 ng mL −1 heat stable FGF2, and either 10 ng mL −1 EGF or 100 nM
Techniques: Immunostaining